Journal: Journal of Cellular and Molecular Medicine

Article Title: Hypoxia‐Induced Metabolic and Functional Changes in Oral CSCs : Implications for Stemness and Viability Modulation Through BNIP3 ‐Driven Mitophagy

doi: 10.1111/jcmm.70400

Figure Lengend Snippet: Hypoxia‐exposed OECM‐1 CSCs exhibit enhanced levels of mitophagy and autophagy. (A) Western blot analysis showing levels of the mitophagy/autophagy markers (PINK1, PARKIN, pPARKIN, LC3I/II, BNIP3, and BNIP3L) in the whole‐cell lysates of normoxia‐ and hypoxia‐exposed OECM‐1 and OECM‐1 CSCs for 6 and 16 h. (B) Representative immunofluorescence images of LC3B staining (quantification presented as number of LC3B puncta/cell) (scale bar, 8.0 μm), co‐localised LC3B and TOMM20 (C), and BNIP3 (D) in cultured cells of OECM‐1 and OECM‐1 CSCs that were either exposed to normoxia or hypoxia for 16 h (scale bar, 25.0 μm). Values are mean ± SD, with replicates performed independently more than three times. Significance is ascribed as * p < 0.5, ** p < 0.01, *** p < 0.01 and **** p < 0.0001 versus OECM‐1 and OECM‐1 CSCs control (normoxia); $ p < 0.05, $$ p < 0.01 and $$$ p < 0.01 versus hypoxia‐exposed OECM‐1 cells.

Article Snippet: p‐PARKIN , 52 kDa , 1:1000 , Rabbit , BiOrbyt , orb312554.

Techniques: Western Blot, Immunofluorescence, Staining, Cell Culture, Control

Journal: Cells

Article Title: Rlip Reduction Induces Oxidative Stress and Mitochondrial Dysfunction in Mutant Tau-Expressed Immortalized Hippocampal Neurons: Mechanistic Insights

doi: 10.3390/cells12121646

Figure Lengend Snippet: Summary of antibody dilutions and conditions used in the immunoblotting analysis of Rlip, synaptic, and mitophagy proteins in mTau HT22 cells transfected with Rlip overexpression and Rlip silencing.

Article Snippet: Parkin , Mouse polyclonal , 1:100 , Novus Biological, Littleton, CO, USA , NBP2-29838.

Techniques: Western Blot, Transfection, Over Expression

Journal: Cells

Article Title: Rlip Reduction Induces Oxidative Stress and Mitochondrial Dysfunction in Mutant Tau-Expressed Immortalized Hippocampal Neurons: Mechanistic Insights

doi: 10.3390/cells12121646

Figure Lengend Snippet: Primary and secondary antibodies used for IF (immunofluorescence).

Article Snippet: Parkin , Mouse polyclonal , 1:100 , Novus Biological, Littleton, CO, USA , NBP2-29838.

Techniques: Immunofluorescence